Home > Sandwich ELISA Kits > Human Plasminogen Activator Inhibitor 2 (PAI-2) ELISA Kit

Product Name:

Human Plasminogen Activator Inhibitor 2 (PAI-2) ELISA Kit

Cat.#:

JL25684

Regulatory Status:

RUO

Alternative:

SERPINB2; PLANH2; Serpin Peptidase Inhibitor Clade B Member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.34ng/mL

Specificity:

This kit recognizes Human PAI-2 in samples. No significant cross-reactivity or interference between Human PAI-2 and analogues was observed.

Sample Type:

Plasma;tissue homogenates;cell lysates;cell culture supernatants and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PAI-2

Plasminogen activator inhibitor-2 (PAI-2) , a serine protease inhibitor of the serpin superfamily, is a coagulation factor that inactivates tissue plasminogen activator and urokinase. PAI-2 exists in two forms, a 6-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PAI-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human PAI-2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-114
1 : 4	Average (%)	94	98	107